How Long Does Glutathione Take to Work & Show Results? Performance Lab Tylenol And Glutathione Invigor Medical Frontiers Crosstalk along the gut liver axis modulates glutathione and cadmium induced hepatotoxicity Yes there is good evidence that acetaminophen (the active ingredient in Tylenol) can deplete glutathione under certain conditions. But whether that matters depends on dose, duration, and individual factors.