Yes there is good evidence that acetaminophen (the active ingredient in Tylenol) can deplete glutathione under certain conditions. But whether that matters depends on dose, duration, and individual factors. Pyroglutamic Acidemia: An Underrecognized and Underdiagnosed Cause of High Anion Gap Metabolic Acidosis A Case Report and Review of Literature Cureus Paracetamol (Acetaminophen) and the Developing Brain Does Acetaminophen Deplete Glutathione? Understanding the Connection Cymbiotika CYMBIOTIKA