Short Note On Carnitine Shuttle: Fatty acids are oxidized in mitochondria, and synthesized in the cytoplasm. Long chain fatty acids must form an active intermediate, fatty acyl CoA, before being oxidized inside mitochondria. Catherine Role of L carnitine in the transportation of long chain fatty acid across the mitochondria membrane. Fatty acid transport into mitochondria by carnitine transporter L Carnitine functions as a cofactor for long chain fatty acids across Download Scientific Diagram