Yes there is good evidence that acetaminophen (the active ingredient in Tylenol) can deplete glutathione under certain conditions. But whether that matters depends on dose, duration, and individual factors. Tylenol's Impact on Glutathione and Liver Damage Is Tylenol Safe During Pregnancy? Understanding Risk Factors Seeking Health Elderly mice with history of acetaminophen intoxication display worsened cognitive impairment and persistent elevation of astrocyte and microglia burden Scientific Reports